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Spatial Transcriptomics: Methods and Applications in R

Dates

  • From Tuesday 6/10/2026 to Thursday 8/10/2026

Speakers

Aurélien Barré, Benjamin Dartigues


Objectives

  • Analyze and manipulate spatially resolved transcriptomics datasets (sequencing and imaging data)
  • Apply methodological concepts specific to spatial data
    (spatial statistics, niche enrichment analysis)
  • Integrate spatial approaches with single-cell RNA-seq analyses for cell annotation and identification

Program

This training course will introduce the essential tools for spatial transcriptomic analysis in R, with a focus on the Seurat package, which enables the manipulation, analysis, and visualization of spatial data. Several other R packages specialized in spatial data analysis will also be presented.

  • Introduction to the concepts of spatially resolved RNA sequencing (sequencing-based and image-based)
  • Importing spatial data into R
  • Data quality control and preprocessing
  • Spatial visualization of gene expression
  • Data normalization
  • Marker identification
  • Data deconvolution for cell identification

Prerequisite

Proficiency in the R language
Must have completed the “R Language: Introduction” course or have equivalent knowledge


Public

Researchers, engineers, and technicians in biology and bioinformatics
Professionals interested in analyzing spatial transcriptomics data or replicating methodologies described in scientific publications